Method for production of Plasmodium causing relapsing malaria

Details Method for production of Plasmodium causing relapsing malaria Method for production of Plasmodium causing relapsing malaria

1996-08-08

2000-07-18

Sayala, Chhaya D.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Protozoa, media therefor

C12N 110

Patent

active

060906146

ABSTRACT:
This invention provides for recycling parasites and maintaining long-term cultures of P. vivax and P. ovale. Preferred conditions include 1) the immediate transfer of parasites to human reticulocytes during the first and second in vitro culture cycles, 2) the use of McCoy's 5A medium modified with L-glutamine containing 25 mM HEPES buffer supplemented with 20% human AB serum, 3) the continual addition of reticulocytes to the culture every 34-44 hours after the beginning of a new culture cycle, and 4) the use of alternate static (growth and differentiation phase) and shaker (invasion phase) culture.

REFERENCES:
patent: 4681899 (1987-07-01), Ohnishi
Renapurkar et al., IRCS. Med Sci., vol. 11(1), p. 7-8, 1983.
Mons et al., Exp. Parasitol., vol. 66(2), p. 183-188, 1988.
Zhou et al., Chinese J. Parasitology & Parasitic Diseases, vol. 9(4), p. -60, 1991.

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