Method for production of native human leukocyte (alpha) interfer

Details Method for production of native human leukocyte (alpha) interfer Method for production of native human leukocyte (alpha) interfer

1998-05-12

2000-12-05

Marx, Irene

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using tissue cell culture to make a protein or polypeptide

435 704, C12P 2104

Patent

active

061565423

ABSTRACT:
The invention relates to a process for the preparation of natural human alfa interferon from human blood by ammonium chloride haemolysis and purification by washing with buffered physiological saline solution, then preparing a suspension in a liquid culture medium and induction by Sendai virus. The process according to the invention is characterized in that leukocytes are separated from human blood, purified in a known way, suspended in a liquid culture medium, then induced by Sendai virus, and the pre-treatment is carried out at 30-40.degree. C., 60-90 minutes after the induction the incubation is continued at 30.degree. C. or at a lower temperature, at the end of incubation further quantity of Sendai virus is added to the suspension, then the cells are separated, and the pH of the supernatant is made acidic.

REFERENCES:
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ATCC Cell Lines and Hybrodomas, 1994, p. 518.
Fierlbeck and Rassner, J. Interferon Res., 9(Suppl. 2), p. S221, 1989.
Boccara et al., J. Interferon Res., 11(Suppl. 1), p. S242, 1991.
Kikuta et al., J. Gen. Virol., 65, p. 837-841, 1984.
Roberts et al., J. Immunol. 123, p. 365-369, 1979.
Saksela et al., Prog. Med. Virol., 30, p. 78-86, 1984.

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