Method for production of L-phenylalanine by recombinant E. coli

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...

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435 691, 435 712, 4352523, 43525233, 4351723, 4353201, 435849, 536 232, 935 14, 935 29, 935 41, 935 43, 935 56, 935 60, 935 61, 935 73, C12P 1522, C12N 1573, C12N 121

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053044754

ABSTRACT:
A novel E. coli (KCCM 10,013) which can produce high yields of L-phenylalanine and a method for production of L-phenylalanine by recombinant E. coli which may be transformed with a novel plasmid pMW16 containing two promoters and a temperature-sensitive repressor for expressing a pheA gene and an aroF gene wherein chorismate mutase p-prephenate dehydratase is coded for by the pheA gene and 3-deoxy-D-arabinoheptulosonate.7-phosphate synthase is coded for by the aroF gene.

REFERENCES:
patent: 5008190 (1991-04-01), Lee et al.
patent: 5030567 (1991-07-01), Lee et al.
Sugimoto, S. et al. "Hyperproduction of Phenylalanine by Escherichia coli: Application of a Temperature-Controllable Expression Vector Carrying the Repressor-Promoter System of Bacteriophage lambda" J. Biotechnology 5:237-253 (1987).

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