Method for production of L-glutamine

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...

Reexamination Certificate

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C435S193000, C435S252300, C435S252320, C536S023200

Reexamination Certificate

active

08067211

ABSTRACT:
A polypeptide comprising an amino acid sequence in which one or more amino acids are deleted, substituted or added in the amino acid sequence of a glutamine synthetase 2 derived from a microorganism belonging to a coryneform bacterium, wherein the coryneform bacterium producing the polypeptide has L-glutamine productivity, a DNA encoding the polypeptide, a recombinant DNA comprising the DNA, a microorganism comprising the DNA or the recombinant DNA, and a process for producing L-glutamine using the microorganism are provided.

REFERENCES:
patent: 6855516 (2005-02-01), Nagai et al.
patent: 2003/0003550 (2003-01-01), Nakamura et al.
patent: 2003/0148474 (2003-08-01), Gusyatiner et al.
patent: 55-148094 (1980-11-01), None
patent: 03-232497 (1991-10-01), None
Nolden, et al., “Glutamine synthetases ofCorynebacterium glutamicum: transcriptional control and regulation of activity”, FEMS Microbiology Letters, vol. 201 (2001) 91-8.
Jakoby, et al., “Nitrogen regulation inCorynebacterium glutamicum: isolation of genes involved and biochemical characterization of corresponding proteins”, FEMS Microbiology Letters, vol. 173 (1999) 303-10.
Hirasawa, et al., “L-Glutamate production by lysozyme-sensitiveCorynebacterium glutamicumltsA mutant strains”, BMC Biotechnology, vol. 1, No. 9 (2001).
Hirasawa, et al., “A Mutation in theCorynebacterium glutamicumltsA Gene Causes Susceptibility to Lysozyme, Temperature-Sensitive Growth, and L-Glutamate Production”, Journal of Bacteriology, vol. 182, No. 10 (2000) 2696-2701.

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