Method for production of human tissue type plasminogen activator

Details Method for production of human tissue type plasminogen activator Method for production of human tissue type plasminogen activator

1989-09-27

1993-02-02

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435212, C12N 964, C12N 948

Patent

active

051837544

ABSTRACT:
A method for the production of human tissue type plasminogen activator (human t-PA) using cells is disclosed. The method comprises producing the human t-PA in parallel with the growth of the cells or after the cells have been grown. To the cell culture bicarbonate ion is added at a concentration equivalent to 3 to 10 g/l of NaHCO.sub.3 based on the true volume of the medium to increase the osmotic pressure of the medium to a range of 350 to 520 milliosmoles/liter.

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patent: 4960702 (1990-10-01), Rice et al.
Grant et al, Thromb. Res. 40(3):393-400, 1985 in Biological Abstracts, vol. 81, No. 10, abstract 89439, 1986.

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