Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing a carotene nucleus
Reexamination Certificate
2006-08-29
2006-08-29
Prouty, Rebecca E. (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing a carotene nucleus
C435S006120, C435S069100, C435S193000, C435S252300, C435S254200, C435S320100, C435S419000, C435S166000, C435S167000, C435S183000, C435S325000, C536S023200
Reexamination Certificate
active
07098000
ABSTRACT:
The present invention provides methods to engineer microorganisms for the production of C30-aldehyde carotenoids. Specifically, various combinations of crtM, sqs, crtN and crtN2 genes fromStaphylococcus aureusandMethylomonassp. 16acan be co-expressed in transformant hosts, leading to the production of diaponeurosporene monoaldehyde, diapocarotene monoaldehyde, and/or diapocarotene dialdehyde. In a preferred embodiment, the genetically engineered pathway is introduced into a strain ofEscherichia colithat has been engineered for the expression of carotenoids, and the C30-carotenoid product is diapocarotene dialdehyde.
REFERENCES:
patent: 5182208 (1993-01-01), Johnson et al.
patent: 5429939 (1995-07-01), Misawa et al.
patent: 5466599 (1995-11-01), Jacobson et al.
patent: 5530188 (1996-06-01), Ausich et al.
patent: 5530189 (1996-06-01), Ausich et al.
patent: 5545816 (1996-08-01), Ausich et al.
patent: 5656472 (1997-08-01), Ausich et al.
patent: 5691190 (1997-11-01), Girard et al.
patent: 5972642 (1999-10-01), Fleno et al.
patent: 6015684 (2000-01-01), Jacobson et al.
patent: 6124113 (2000-09-01), Hohmann et al.
patent: 2003/0182687 (2003-09-01), Cheng et al.
Umeno et al. Evolution of the C30 Carotenoid synthase crtM for function in a C40 pathway. Journal of Bacteriology, Dec. 2002, p. 6690-6699.
Alex Raising . 4, 4′diaponeurosporene desaturase: Catalytic properties of an enzyme from the C30 Carotenoid□□pathway ofStaphylococcus aureus. Journal of Bacteriology, Oct. 1999, p. 6184-187.
Marshall et al, Proposed pathway of Terpenoid Carotenoid biosynthesis inStaphylococcus aureus: Evidence from a study of Mutants J. Bacteriol. 147 No. 3: pp. 914-919, 1981).
Nelis and Leenheer, Microbial sources of carotenoid pigments used in foods and feeds, Appl. Bacteriol. 70: pp. 181-191, 1991.
Armstrong, Eubacteria Show Their True Colors: Genetics of Carotenoid Pigment Biosynthesis from Microbes to Plants, J. Bact. 176: pp. 4795-4802, 1994.
Armstrong, Genetics of Eubacterial Carotenoid Biosynthesis: A Colorful Tale, Annu. Rev. Microbiol. 51: pp. 629-659, 1997.
Farmer et al., Precursor Balancing for Metabolic Engineering of Lycopene Production in Escherichia coli, Biotechnol. Prog. 17: pp. 57-61, 2001.
Wang et al., Directed Evolution of Metabolically Engineered Escherichia coli for Carotenoid Production, Biotechnol. Prog. 16: pp. 922-926, 2000.
Misawa et al., Metabolic engineering for the production of carotenoids in non-carotenogenic bacteria and yeasts, J. Biotchenol. 59: pp. 169-181, 1998.
Shimada et al., Increased Carotenoid Production by the Food YeastCandida utilisthrough Metabolic Engineering of the Isoprenoid Pathway, Appl. Environ. Microbiol. 64:pp. 2676-2680, 1998.
Albrecht et al., Metabolic Engineering of the terpenoid biosynthetic pathway of Escherichia coli for production of the carotenoids β-carotine and zeaxanthin, Biotechnol. Lett. 21: pp. 791-795, 1999.
Miura et al., Production of the Carotenoids Lycopene, β-Carotene, and Astaxanthin in the Food Yeast Candida utilis, Appl. Environ. Microbiol. 64:pp. 1226-1229.
Z. Naturforsch 34c: New C30-Carotinoic Acid Glucosyl Esters fromPseudomonas rhodos, pp. 181-185, 1979.
Z. Naturforsch 37c: On the Biosynthesis of C30-Carotenoic Acid Glucosyl Esters inPseudomonas rhodos. Analysis of car-Mutants, pp. 758-760, 1982.
Taylor et al., Triterpenoid Carotenoids and Related Lipids, J. Biochem. 139: pp. 751-760, 1974.
Taylor, Bacterial Triterpenoids, Microbiol. Rev. 48: pp. 181-198, 1984.
Takaichi et al., The major carotenoid in all known species of heliobacteria is the C30carotenoid 4,4′-diaponeurosporene, not neurosporene, Arch. Microbiol. 168, pp. 277-281, 1997.
Marshall et al, Proposed Pathway of Triterpenoid Carotenoid Biosynthesis inStaphylococcus aureus: Evidence from a Study of Mutants, , J. Bacteriol. 147 No. 3: pp. 900-913, 1981.
Raisig et al.,Functional properties of diapophytoene and related desaturases of C30and C40carotenoid biosynthetic pathway, Biochim. Biophys. Acta 1533: 164-170, 2001.
Xiong et al., Tracking molecular evolution of photosynthesis by characterization of a major photosynthesis gene cluster fromHeliobacillus mobilis, Proc. Natl. Acad. Sci. USA 95: 14851-14856, 1998.
Takami et al., Genome sequence ofOceanobacillus iheyensisisolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments, Nucleic Acids Res., 30: pp. 3927, 3935, 2002.
Marshall et al., Proposed Pathway of Triterpenoid Biosynthesis inStaphylococcus aureus: Evidence from a Study of Mutants, J. Bacteriol. 147:pp. 914-919, 1981.
Ingram et al., Effect of Selected Aldehydes on the Growth and Fermentation of EthanologenicEscherichia coli, Biotechnol. Bioeng. 65: 24-33, 1999.
Marnett et al., Mtagenicity inEscherichia coliof the major DNA adduct derived from the endogenous mutagen malondialdehyde, Proc. Natl. Acad. Sci. USA 94: 8652-8657, 1997.
Mee et al., Formaldehyde is a bacterial mutagen in a range ofSalmonellaandEscherichiaindicator strains, Mutagenesis 8: pp. 577-581, 1993.
Kawazoe et al., Mutagenic characteristics of formaldehyde on bacterial systems, Mutat. Res. 156: pp. 153-161, 1985.
Cheng Qiong
Tao Luan
E. I. du Pont de Nemoure and Company
Gebreyesus Kagnew
Prouty Rebecca E.
LandOfFree
Method for production of C30-aldehyde carotenoids does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for production of C30-aldehyde carotenoids, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for production of C30-aldehyde carotenoids will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3663547