Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1984-03-15
1987-12-08
Hazel, Blondel
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
4351722, 43524026, C12P 2100, C12N 1500
Patent
active
047118423
ABSTRACT:
A method of producing biologically active substances using a hybrid cell line obtained by fusing (1) a human tumor cell line which does not grow in a medium containing aminopterin or/and azaserine or cell line derived therefrom with (2) a human non-tumor macrophage. The human tumor macrophage cell line may be either a thymidine kinase-deficient cell line of U-937 or a thymidine kinase-deficient cell line of THP-1, the non-tumor macrophage may be alveolar macrophage, splenic macrophage, peripheral blood monocyte, peritoneal macrophage, hepatic macrophage, placental macrophage, or thymic macrophage, and the biologically active substance produced is a tumoricidal substance or interleukin-1.
REFERENCES:
patent: 4438032 (1984-03-01), Golde et al.
patent: 4529694 (1985-07-01), Lazarus
patent: 4544632 (1955-10-01), Yamamura et al.
Miyamura et al., J. Antibiotics, vol. 36, No. 6, 1983, pp. 684-687.
Smith et al., J. Chem. Soc., Perkins Trans. I, vol. 22, p. 2811, 1972.
Bloom et al., Ed., "In Vitro Methods in Cell-Modulated Immunity", p. 353.
Matthew, Immunology, vol. 44, pp. 135-142, 1981.
Taniyama Tadayoshi
Yoshida Koichi
Asahi Kasei Kogyo Kabushiki Kaisha
Hazel Blondel
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