Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2005-11-08
2010-12-28
Monshipouri, Maryam (Department: 1656)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
Reexamination Certificate
active
07858343
ABSTRACT:
A heterologous protein is produced by a method in which yeast-type host cells are prepared, which, in addition to the at least one endogenous homologous DNA sequence coding for a first calnexin, includes at least one recombinant DNA sequence coding for the protein and at least one additional recombinant DNA sequence coding for at least one second calnexin. The protein in the host cell represents a heterologous secretable protein. The host cells are induced to express the heterologous protein via the at least one sequence coding for the protein and to overexpress calnexins by the at least one additional sequence coding for the at least one second calnexin. The host cells are then induced to secrete the heterologous protein. The secreted heterologous protein is then separated off.
REFERENCES:
Klabunde: “Koproduktion Pharmazeutischer Proteine and Hilfsfaktoren zur Optimierung mikrobieller Expressionssysteme bei Beschrankung auf ein einziges integratives Vektorsystem”, 2003, Universitats- Und Landesbibliothek Dusseldorf, Dusseldorf, Deutschland.
Punt P. J et al: “Filamentous Fungi as cell factories for heterologous protein production”, Trends in Biotechnology, Elsevier Publications, Cambridge, GB, Bd. 20, Nr. 5, May 1, 2002, pp. 200-206.
De Virgilio Claudio et al: “CNE1, aSaccharomyces cerevisiaehomologue of the genes encoding mammalian calnexin and calreticulin”, Yeast, Bd. 9, Nr. 2, 1993, pp. 185-188.
Higgins M K et al: “Calnexin co-expression and the use of weaker promoters increase the expression of correctly assembled Shaker potassium channel in insect cells”, Biochimica Et Biophysica Acta. Biomembranes, Amsterdam, NL, Bd. 1610, Nr. 1, Feb. 17, 2003, pp. 124-132.
Marechal Alexandre et al: “Cell viability and secretion of active proteins inSchizosaccharomyces pombedo not require the chaperone function of calnexin”, The Biochemical Journal, Jun. 1, 2004, Bd. 380, Nr. Pt 2, Jun. 1, 2004, pp. 441-448.
Arima et al: “Enhanced secretion of hydrophobic peptide fused lysozyme by the introduction of N-glycosylation signal and the disruption of calnexin gene inSaccharomyces cerevisiae”, FEBS Letters 440 (1998), pp. 89-92.
Klabunde et al: “Integration of heterologous genes in several yeast species using -vectors containing aHansenula polymorpha-derived rDNA-targeting element”, FEMS Yeast Research 4 (2003), pp. 185-193.
Gellissen, G. (ed), “Hansenula polymorpha—biology and applications”, Wiley-VCH, Weinheim 2002, Chapter 8, Suckow et al., “The expression platform based onH. polymorphastrain RB11 and its derivatives—history, status and perspectives” pp. 105-123; and Chapter 17, Degelmann, “Methods” pp. 285-335.
Degelmann Adelheid
Gellissen Gerd
Hollenberg Cornelis
Klabunde Jens
Artes Biotechnology GmbH
Fay Sharpe LLP
Monshipouri Maryam
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