Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1994-11-03
1996-06-11
Walsh, Stephen G.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435244, 435803, 935 38, 530359, 530380, 530420, 530422, C12N 138, C12P 2100, C07K 130, C07K 14775
Patent
active
055254721
ABSTRACT:
Applicants describe methods for purifying human ApoE or analog thereof from recombinant bacterial cells with minimal protein aggregation and degradation during the purification process. The invention involves the addition of neutralized fatty acids to the medium during cell disruption and the use of a non-ionic detergent during the purification process. Additionally applicants describe a method for increasing the production of ApoE or analog thereof in a bacterial host by adding to the culture medium neutralized fatty acids, fatty acid precursors, triglycerides, triglyceride precursors or acetate. Pharmaceutical and diagnostic uses of the ApoE analog are described.
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Bartfeld Daniel
Fischer Meir
Greenman Benjamin
Lifshitz Ruth
Bio-Technology General Corp.
Walsh Stephen G.
White John P.
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