Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using tissue cell culture to make a protein or polypeptide
Patent
1991-11-12
1993-09-07
Furman, Keith C.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Using tissue cell culture to make a protein or polypeptide
424 88, 424 89, 435 693, 530395, 530412, 530414, 530415, 530416, 530417, 530806, 935 65, C07K 326, C07K 328, C07K 320, C07K 1514
Patent
active
052428125
ABSTRACT:
Processes are provided for producing purified, hepatitis B surface antigen particles in mammalian cells which comprise culturing mammalian cells which produce the particles in a culture medium supplemented with a serum free of high molecular weight contaminant proteins and recovering the purified, hepatitis B surface antigen particles.
Removal of molecules having a molecular weight greater than about 3.times.10.sup.5 daltons by prefractionation, for example, allows cells to be grown in culture media containing high levels of fetal calf serum, removes high molecular weight contaminant proteins which may be inhibitory to cell growth and simplifies purification of HBsAg since high molecular weight contaminant proteins are the major contaminants removed by purification processes.
REFERENCES:
patent: 4349539 (1982-09-01), Wampler
patent: 4558011 (1985-12-01), Brzosko et al.
patent: 4738926 (1988-04-01), Hamada et al.
Lee et al. 1987, Journal of the Chinese Biochemical Society 16(1): 7-14.
Molnar-Kimber et al. 1988, J. Virology 62(2): 407-416.
Zwerner et al. 1979, Methods in Enzymology, vol. 58: 221-229.
Bio-Technology General Corp.
Furman Keith C.
White John P.
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