Method for producing vitronectin

Details Method for producing vitronectin Method for producing vitronectin

1988-03-22

1991-12-31

Wax, Robert A.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

435101, 435103, 530350, 530380, 530395, 530412, 530415, 530417, 530419, 935 9, C07K 320

Patent

active

050773933

ABSTRACT:
This invention deals with a simple and efficient method for producing vitronectin from biological materials which include vitronectin. More specifically, this invention deals with a method for producing vitronectin by binding vitronectin in biological materials to immobilized glycosaminoglycans in the presence of protein denaturing agent, especially urea.

REFERENCES:
Suzuki et al.; Journal of Biological Chemistry; Domien Structure of Ultronectin; pp. 15307-15314 (1984).
B. Dahlback et al., Biochemistry, vol. 24, No. 9, pp. 2368-2374 (1985).
E. G. Hayman et al., Proc. Natl. Acad. Sci. U.S.A., vol. 80, pp. 4003-4007 (1983).
Takao Akama et al., J. Biochem., vol. 100, No. 5, pp. 1343-1351 (1986).
D. W. Barnes et al., The Journal of Biological Chemistry, vol. 258, No. 20, pp. 12548-12552 (1983).
Masao Hayashi et al., J. Biochem., vol. 98, No. 4, pp. 1135-1138 (1985).

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