Method for producing vitamin B12 from hydrogen-metabolizing...

Details Method for producing vitamin B12 from hydrogen-metabolizing... Method for producing vitamin B12 from hydrogen-metabolizing...

2006-03-28

2006-03-28

Lilling, Herbert J. (Department: 1651)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C095S055000, C096S004000, C210S500260, C427S255110, C427S255120

Reexamination Certificate

active

07018815

ABSTRACT:
Mesophilic methane bacteria obtained from digested sludge are acclimatized in an H2/CO2medium and the acclimatized methane bacteria are grown on a support inclusive of inorganic nutritional salts of trace metal elements by using an immobilized bed bioreactor. The grown bacteria are allowed to metabolize a mixed gas, prepared by adding hydrogen to at least one of coal gas and biogas, into methane and at the same time, cobalamin contained in the fermented liquor is recovered as an extracellular product in the form of cyanocobalamin by using potassium cyanide to thereby produce vitamin B12efficiently in a high content and high yield.

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