Method for producing type IV collagen

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Scleroproteins – e.g. – fibroin – elastin – silk – etc.

Reexamination Certificate

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C514S002600

Reexamination Certificate

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07741441

ABSTRACT:
An object of the invention is to provide type IV collagen without contamination by other proteins and without degradation or denaturation. The present invention provides a type IV collagen which is derived and extracted from lens capsules without the use of an enzyme and has a minimum molecular weight of 160 to 180 kDa measured by SDS-PAGE under reduced conditions.

REFERENCES:
patent: 6060255 (2000-05-01), Shibuya et al.
Aline Sionkowska, “Thermal stability of UV-irradiated collagen in bovine lens capsules and in bovine cornea”, Journal of Photochemistry and Photobiology B: Biology 80(2): 87-92 (2005).
Sage et al., The Journal of Biological Chemistry, vol. 254, No. 19, Oct. 10, 1979, pp. 9893-9900.

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