Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide contains a tissue – organ – or cell...
Patent
1999-04-14
2000-07-04
Fox, David T.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide contains a tissue, organ, or cell...
800278, 800289, 800294, 800307, 800309, 514783, 4354301, 435431, 435469, 4352523, 4353201, 4352522, 536 232, 536 236, 536 231, C12N 504, C12N 1529, C12N 1582, C07H 2104, A01H 500
Patent
active
060841524
ABSTRACT:
The invention relates to a transgenic plant that produces high levels of superoxide dismutase (SOD) and to a method for producing the transgenic plant. The hypocotyl section of seedlings is co-cultured with Agrobacterium transformant and regenerated by adventitious shoot induction and by root induction, where the Agrobacterium transformant contains an expression vector that comprises the promoter of a fruit-dominant ascorbate oxidase gene, an SOD gene isolated from cassava, and an herbicide-resistant bar gene. The present invention also relates to a method for inducing adventitious shoot from hypocotyl sections in plant tissue culture, thus providing a method for the efficient production of transgenic plants maintaining higher SOD activity in fruits. Therefore, the SOD transgenic cucumber of the present invention can be used for cosmetics, additives in functional foods, and medicines as well as having tolerance to herbicides and environmental stresses.
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Ohkawa et al. Proc. Natl. Acad. Sci. USA, vol. 86, pp. 1239-1243, Feb. 1989.
Randy D. Allen, "Dissection of Oxidative Stress Tolerance Using Transgenic Plants", Plant Physiol. vol. 107: 1049-1054 (1995).
Kim Jae-Whune
Kwak Sang Soo
Kwon Suk Yoon
Lee Haeng-Soon
Fox David T.
Ibrahim Medina A.
Korea Institute of Science and Technology
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