Method for producing tissue formation and tissue culture kit

Details Method for producing tissue formation and tissue culture kit Method for producing tissue formation and tissue culture kit

1997-05-19

2000-04-18

Lankford, Jr., Leon B.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

435347, 435373, 435395, 435397, 435398, 424484, 424488, 4283105, 4283166, C12N 500

Patent

active

060514256

ABSTRACT:
A matrix for tissue culture comprising two kinds of sponges having at least one different physical property and/or at least one different chemical property; a method for culturing tissue using said matrix for tissue culture comprising inoculating and culturing first cell on a first sponge, laminating a second sponge thereon, and inoculating and culturing second cell on said second sponge; a method for fixing a cultured tissue comprising placing a cultured tissue in gelatin solution solated by elevating temperature, lowering temperature to gelatinize gelatin to fix the cultured tissue by said gelatinated gelatin; and an artificial skin fixed comprising dermis layer fixed by gelatin and epidermis layer laminated on the dermis layer.

REFERENCES:
patent: 4485096 (1984-11-01), Bell
patent: 4835102 (1989-05-01), Bell et al.
patent: 4841962 (1989-06-01), Berg et al.
patent: 5024841 (1991-06-01), Chu et al.
patent: 5350583 (1994-09-01), Yoshizato et al.
"Composite Skin Substitute Composed of Cultured Keratinocytes and Fibroblasts Combined in Collagen Matrix" Ohtake et al., J. Jpn. P.R.S., 10: 165-180, 1990.
"Characterization of a Three Dimensional Co-Culture of Neonatal Human Fibroblasts and Keratinocytes" Slivka, et al. The Journal of Investigative Dermatology, 96: 544A, 1991.

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