Method for producing the SSPI restriction endonuclease and methy

Details Method for producing the SSPI restriction endonuclease and methy Method for producing the SSPI restriction endonuclease and methy

1994-10-06

1996-05-14

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435193, 43525233, 4353201, 536 232, C12N 922, C12N 1555

Patent

active

055166784

ABSTRACT:
The present invention is directed to a method for cloning and producing the SspI restriction endonuclease by 1) introducing the restriction endonuclease gene from Sphaerotilus species into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the vector encoding and expressing the SspI restriction endonuclease, and 3) purifying the SspI restriction endonuclease from the fermented host which contains the vector encoding and expressing the SspI restriction endonuclease activity.

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