Method for producing the NLAVI restriction endonuclease and meth

Details Method for producing the NLAVI restriction endonuclease and meth Method for producing the NLAVI restriction endonuclease and meth

1988-07-28

1991-12-24

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

4353201, 4352523, 4351723, 536 27, C12N 922, C12N 1555

Patent

active

050752324

ABSTRACT:
The present invention is directed to a method for cloning and producing the NlaIV restriction endonuclease by 1) introducing the restriction endonuclease gene from N. lactamica NRCC 2118 into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the vector encoding and expressing the NlaIV restriction endonuclease, and 3) purifying the NlaIV restriction endonuclease from the fermented host which contains the vector encoding and expressing the NlaIV restriction endonuclease activity.

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