Method for producing the Nde I restriction endonuclease and meth

Details Method for producing the Nde I restriction endonuclease and meth Method for producing the Nde I restriction endonuclease and meth

1988-05-19

1992-08-18

Patterson, Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

43525233, 4353201, 435193, 536 27, 935 29, 935 73, 935 80, 935 82, C12N 922, C12N 121, C12N 1552

Patent

active

051399423

ABSTRACT:
The present invention is directed to a method for cloning and producing the Nde I restriction endonuclease by 1) introducing the restriction endonuclease gene from Neisseria denitrificans into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the vector encoding and expressing the Nde I restriction endonuclease, and 3) purifying the Nde I restriction endonuclease from the fermented host which contains the vector encoding and expressing the Nde I restriction endonuclease activity.

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