Method for producing the Hpa I restriction endonuclease and meth

Details Method for producing the Hpa I restriction endonuclease and meth Method for producing the Hpa I restriction endonuclease and meth

1989-10-13

1994-03-29

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435193, 43525233, 4353201, 536 232, C12N 922, C12N 1555

Patent

active

052984044

ABSTRACT:
The present invention is directed to a method for cloning and producing the Hpa I restriction endonuclease by 1) introducing the restriction endonuclease gene from Haemophilus parainfluenzae into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the vector encoding and expressing the Hpa I restriction endonuclease, and 3) purifying the Hpa I restriction endonuclease from the fermented host which contains the vector encoding and expressing the Hpa I restriction endonuclease activity.

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