Method for producing the Hinc II restriction endonuclease and me

Details Method for producing the Hinc II restriction endonuclease and me Method for producing the Hinc II restriction endonuclease and me

1989-03-15

1991-05-14

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435199, 43525233, 4353201, 536 27, 935 24, 935 73, 935 80, C12N 1552, C12N 922, C12N 121

Patent

active

050155814

ABSTRACT:
The present invention is directed to a method for cloning and producing the Hinc II restriction endonuclease by (1) introducing the restriction endonuclease gene from Haemophilus influenzae Rc into a host whereby the restriction gene is expressed; (2) fermenting the host which contains the vector encoding and expressing the Hinc II restriction endonuclease, and (3) purifying the Hinc II restriction endonuclease from the fermented host which contains the vector encoding and expressing the Hinc II restriction endonuclease activity.

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