Method for producing the HhaI restriction endonuclease and methy

Details Method for producing the HhaI restriction endonuclease and methy Method for producing the HhaI restriction endonuclease and methy

1987-12-17

1991-03-12

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435199, 43525233, 4353201, 536 27, 935 29, 935 73, 935 80, C12N 1552, C12N 922, C12N 121

Patent

active

049992937

ABSTRACT:
The present invention is directed to a method for cloning and producing the HhaI restriction endonuclease by (1) introducing the restriction endonuclease gene from Haemophilus haemolyticus ATCC 10014 into a host whereby the restriction gene is expressed; (2) fermenting the host which contains the vector encoding and expressing the HhaI restriction endonuclease, and (3) purifying the HhaI restriction endonuclease from the fermented host which contains the vector encoding and expressing the HhaI restriction endonuclease activity.

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