Method for producing the FnuDI restriction endonuclease and meth

Details Method for producing the FnuDI restriction endonuclease and meth Method for producing the FnuDI restriction endonuclease and meth

1987-12-17

1991-01-29

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

43525233, 4353201, 4351723, 435 29, 435 73, 435 80, 435 82, 536 27, C12N 922, C12N 121, C12N 1552

Patent

active

049886207

ABSTRACT:
The present invention is directed to a method for cloning and producing the FnuDI restriction endonuclease by (1) introducing the restriction endonuclease gene from F. nucleatum D into a host whereby the restriction gene is expressed; (2) fermenting the host which contains the vector encoding and expressing the FnuDI restriction endonuclease, and (3) purifying the FnuDI restriction endonuclease from the fermented host which contains the vector encoding and expressing the FnuDI restriction endonuclease activity.

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