Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1987-12-17
1991-01-29
Weimar, Elizabeth C.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
43525233, 4353201, 4351723, 435 29, 435 73, 435 80, 435 82, 536 27, C12N 922, C12N 121, C12N 1552
Patent
active
049886207
ABSTRACT:
The present invention is directed to a method for cloning and producing the FnuDI restriction endonuclease by (1) introducing the restriction endonuclease gene from F. nucleatum D into a host whereby the restriction gene is expressed; (2) fermenting the host which contains the vector encoding and expressing the FnuDI restriction endonuclease, and (3) purifying the FnuDI restriction endonuclease from the fermented host which contains the vector encoding and expressing the FnuDI restriction endonuclease activity.
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Van Cott Elizabeth M.
Wilson Geoffrey G.
Conlin David G.
New England Biolabs Inc.
Patterson Charles L.
Weimar Elizabeth C.
Williams Gregory D.
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