Method for producing the Eag I restriction endonuclease and meth

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435199, 4352523, 4353201, 435193, 43525233, 536 27, 935 29, 935 73, 935 80, 935 82, C12N 1554, C12N 7555, C12N 120

Patent

active

049961519

ABSTRACT:
The present invention is directed to a method for cloning and producing the Eag I restriction endonuclease by (1) introducing the restriction endonuclease gene from E. agglomerans ATCC into a host whereby the restriction gene is expressed; (2) fermenting the host which contains the vector encoding and expressing the Eag I restriction endonuclease, and (3) purifying the Eag I restriction endonuclease from the fermented host which contains the vector encoding and expressing the Eag I restriction endonuclease activity.

REFERENCES:
patent: 6707079 (1988-07-01), Wilson
Chandrasegaran, S. et al., (1987) Structure and Expression, vol. I, Sarma et al. (eds.), Adenine Press, 149-156.
Brooks, J. E. (1987) Met. Enz., vol. 152, 113-129.
Sohail, A. et al. (1987) Pakistan J. Zool. 19(4), 371-391.
Kaue, L. et al. (1986) Acta Microbiologica Polonica 35(3/4), 317-320.
Mann et al., Gene 3: 97-112, 1978.
Kosysh et al., Molec. Gen. Genet. 178: 717-719, 1980.
Walder et al., Proc. Nat. Acad. Sci. U.S.A. 78: 1503-1507, 1981.
Bougueleret et al., Nucleic Acids Res. 12: 3659-3676, 1984.
Gingeras & Brooks, Proc. Natl. Acad. Sci. U.S.A. 80: 402-406, 1983.
Theriault & Roy, Gene 19: 355-359, 1982.
Blumenthal et al., J. Bacteriol. 164: 501-509, 1985.
Kiss et al., Nucleic Acids Res. 13: 6403-6421, 1985.
Szomolanyi et al., Gene 10: 219-225, 1980.
Janulaitis et al., Gene 20: 197-204, 1982.
Kiss & Baldauf, Gene 21: 111-119, 1983.
Walder et al., J. Biol. Chem. 258: 1235-1241, 1983.
Raleigh & Wilson, Proc. Natl. Acad. Sci., U.S.A. 83: 9070-9074, 1986.
Maniatis et al., Molecular Cloning; A Laboratory Manual; Cold Spring Harbor Laboratories, New York; pp. 368-369, 1982.
Greene, P. J. et al. (1981) J. Biol. Chem., 256 (5), 2143-2153.
Newman, A. K. et al. (1981) J. Biol. Chem., 256(5), 2131-2139.
Schoner, B. et al. (1983) Gene 24, 227-238.
Walder, R. Y. et al. (1984) J. Biol. Chem., 259(12), 8015-8026.
Kauc, L. et al. (1987) Chem Abst. 107: 19790g.
Sohail, A. et al. (1989) Chem. Abst. 111: 35574c.
Butkus, V. V. et al. (1985) Chem. Abst. 103: 137590g.
Kessler, C. et al. (1985) Gene 33, 1, 22, 97, 98.
Wilson, G. C. (1988) Trends in Genetics 4(11), 314-318.
Lunnen, K. P. et al. (1988) Gene 24, 25-32.
Borck, K. et al. (1976) Molec. Gen. Genet. 146, 199-207.
Roberts, R. J. (1989) Nuc. Acids Res. 17 (Suppl.), 1347-1387.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for producing the Eag I restriction endonuclease and meth does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for producing the Eag I restriction endonuclease and meth, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for producing the Eag I restriction endonuclease and meth will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-293693

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.