Method for producing the DdeI restriction endonuclease and methy

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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43525233, 4353201, 435193, 536 232, C12N 922, C12N 1555

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active

053546806

ABSTRACT:
A two-step method for cloning the DdeI restriction modification system is provided which comprises introducing the DdeI modification or methylase gene into a host cell and expressing the gene to protect the DdeI host cell followed by introducing the DdeI restriction or endonuclese gene into the host.

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