Method for producing the AFL II restriction endonuclease and met

Details Method for producing the AFL II restriction endonuclease and met Method for producing the AFL II restriction endonuclease and met

1989-11-20

1991-07-09

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435199, 43525233, 4353201, 536 27, 935 29, 935 73, 935 80, C12N 1552, C12N 922, C12N 121

Patent

active

050305697

ABSTRACT:
The present invention is directed to a method for cloning and producing the Afl II restriction endonuclease by 1) introducing the restriction endonuclease gene from Anabaena flos-aquae into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the plasmid encoding and expressing the Afl II restriction endonuclease activity, and 3) purifying the Afl II restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the Afl II restriction endonuclease activity.

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