Method for producing the ACCI restriction endonuclease and methy

Details Method for producing the ACCI restriction endonuclease and methy Method for producing the ACCI restriction endonuclease and methy

1987-12-17

1991-04-02

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435199, 43525233, 4353201, 536 27, 935 29, 935 73, 935 80, C12N 1552, C12N 922, C12N 121

Patent

active

050046918

ABSTRACT:
The present invention is directd to a method for cloning and producing the AccI restriction endonuclease by (1) introducing the restriction endonuclease gene from into a host whereby the restriction gene is expressed; (2) fermenting the host which contains the plasmid encoding and expressing the AccI restriction endonuclease activity, and (3) purifying the AccI restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the AccI restriction endonuclease activity.

REFERENCES:
Greene, P. J. et al., (1981), J. Biol. Chem., 256(5), 2143-2153.
Newman, A. K., et al., (1981), J. Biol. Chem. 256(5), 2131-2139.
Schoner, B., et al., (1983), Gene 24, 227-236.
Walder, R. Y., et al., (1984), J. Biol. Chem. 259(12), 8015-8026.
Roberts, R. J., (1984), Nucleic Acids Res. 12, r167, r168, r204.
Lunnen, K. D., et al., (1988), Gene 74, 25-32.
Wilson, G. G., (1988), Gene 74, 281-289.
Wilson, G. G., (1988), Trends in Genetics 4(11), 314-318.

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