Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide confers resistance to heat or cold
Reexamination Certificate
1998-06-26
2004-06-29
Mehta, Ashwin (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide confers resistance to heat or cold
C435S419000, C435S468000, C800S298000, C800S306000, C800S320000
Reexamination Certificate
active
06756525
ABSTRACT:
This application is the national phase under 35 U.S.C. §371 of prior PCT International Application No., PCT/JP96/03873, which has an International filing date of Dec. 27, 1996, which designated the United States of America, the entire contents of which are hereby incorporated by reference.
FIELD OF THE INVENTION
The present invention relates to a method for producing plants with novel properties, more specifically, a method for producing temperature-tolerant plants which are highly resistant to environmental stress.
PRIOR ART
Many organisms adapt themselves to severe environmental stress by synthesizing and accumulating a specific compound called “compatible solute” in their cytoplasm to protect themselves against such stress. Environmental stress to which organisms have been shown to adapt themselves by such a mechanism include salts (Imhoff et al., FEMS Microbiol. Rev. 39:57-66, 1986; Mackay et al., J. Gen. Microbiol. 130:2177-2191, 1984; Rhodes and Hanson, Annu. Rev. Plant Physiol. Plant Mol. Biol. 44:357-384, 1993), dehydration (Yancy et al., Science 217:1214-1222, 1982) and low temperatures (Ko et al., J. Bacteriol. 176:426-431, 1994).
Among those compatible solutes, glycine betaine (hereinafter referred to as betaine) is widely distributed in higher plants (Robinson and Jones, Aust. J. Plant Physiol. 13:659-668, 1986), bacteria (Csonka, Microbiol. Rev. 53:121-147, 1989) and animals (Garcia-Perez and Burg, Physiol. Rev. 71:1081-1115, 1991; Lever et al., Biochim. Biophys. Acta. 1200:259-264, 1994). As shown in
FIG. 1
, betaine is a bipolar compound having a positive charge and a negative charge in its molecules (Rhodes and Hanson, Annu. Rev. Plant Physiol. Plant Mol. Biol. 44:357-384, 1993). A long discussion regarding the physiological functions of betaine has suggested that betaine may protect cells by maintaining an osmotic balance with the environment (Robinson and Jones, Aust. J. Plant Physiol. 13:659-668, 1986) and that betaine may stabilize higher-order structures of proteins (Bernard et al., Acad. Sci. 111, 307:99-104, 1988; Papageorgiou and Murata, Phtosynth. Res. 44:243-252, 1995). However, betaine is not exclusively synthesized in cells under salt stress or dehydration stress. Thus, it could not be concluded that betaine has a direct effect on the protection of cells against such stress.
In
Escherichia coli
and spinach (
Spinacia oleracea
), betaine is biosynthesized from choline via two steps of oxidation as shown in FIG.
1
. On the other hand, choline oxidase obtained from the gram-positive soil bacterium Arthrobacter globiformis can oxidize choline to betaine in one-step oxidation reaction (Ikuta, S. et al., J. Biochem. 82:1741-1749, 1977).
In an attempt to study a direct effect of betaine, we isolated the coda gene encoding a novel choline oxidase which catalyzes oxidation from choline to betaine (Japanese Society of Plant Physiologist, Annual meeting of 1994, the 34th Symposium held Mar. 28-30, 1994) and integrated it into cells of the cyanobacterium strain Synechococcus PCC7942 and brassicaceous plants, thus succeeded in obtaining salt-tolerant and/or osmotolerant plants (Japanese Patent Application No. 106819/95). This confirmed that betaine functions to protect organisms against salt stress.
However, no report has shown that betaine confers temperature tolerance on plants or bacteria.
It is an object of the present invention to produce plants that are tolerant to environmental changes such as high temperatures or low temperatures by gene recombination techniques.
DISCLOSURE OF THE INVENTION
As a result of careful study to solve the above problems, we succeeded in obtaining temperature-tolerant plants by integrating and expressing a gene encoding choline oxidase in cyanobacteria, brassicaceous plants and gramineous plants.
Accordingly, the present invention provides a method for producing temperature-tolerant plants, which comprises transforming a plant with a recombinant vector carrying a gene encoding choline oxidase.
The present invention also provides temperature-tolerant plants produced by said method or progenies thereof having the same properties.
REFERENCES:
patent: 6281412 (2001-08-01), Murata
patent: 818138 (1998-01-01), None
patent: WO 9414970 (1994-07-01), None
patent: WO 9826081 (1998-06-01), None
M. D. Mamedov et al.,FEBS Lett., vol. 294, No. 3, pp. 271-274 (1991).
N. Murata et al.,FEBS Lett., vol. 296, No. 2, pp. 187-189 (1992).
B. Rathinasabapathi et al.,Planata, 193:155-162 (1994).
K. Holmström et al.,The Plant Journal, 6(5), pp. 749-758 (1994).
Pathcharaporn, et al., Chemical Abstracts, vol. 126, Jun. 2, 1997, XP002087537.
Murata N. et al., Phys. Stresses Plants: Genes Their Prod. Tolerance, pp. 55-63, Sep. 1995, XP002062848.
Mamedov M. et al., Database Biosis Biosciences Information Service, (abstract), 1993, XP002087538.
Deshnium et al., Embl Accession, Feb. 27, 1995, XP002087232.
Holmstroem et al., The Plant Journal, vol. 6, 1994, XP002034433 pp 749-758.
Kishitani et al., Database Biosis Biosciences Information Service, 1994, XP002087539.
Rathinasabapathi et al., Planta, vol. 193, 1994, XP002034434 pp 155-162.
Palva et al., Sep. 24-27, 1995 pp. 187-198, XP002044597.
Paleg et al., Biological Abstracts, vol. BA72, (Abstract) 1981, XP002087536.
Zhao et al., Database Biosis Biosciences Info. Service, (Abstract), 1992, XP002087540.
Ko et al., Database Biosis Biosciences Info. Service, (Abstract), 1994, XP002087541.
Rhodes et al., Annu. Rev. Plant Physiol. Plant Mol. Biol., 1993 vol. 44, pp. 357-384, XP002087532.
Kodama et al., Plant Physiology, vol. 105, pp. 601-605, Jan. 1, 1994, XP002001002.
Lee et al., Plant Journal, vol. 8, pp. 603-612, Oct. 1, 1995, XP002003021.
Derwent Publications Ltd., Database WP1, Oct. 15, 1996, XP002062849.
Wada et al., Nature, vol. 347, Sep. 13, 1990, pp. 200-203, XP002087535.
Patcharaporn Deshnium et al.,Plant Molecular Biology, vol. 29, pp 897-907, 1995.
Mika Nomura et al.,Plant Physiol, vol. 107, pp. 703-708, 1995.
Kevin L. Rozwadowski et al.,Journal of Bacteriology, vol. 173, No. 2, pp. 472-478, 1991.
Bartels et al. Plant, Cell and Environment, vol. 17, pp. 659-667, 1994.*
Deshnium et al. Plant Molecular Biology, vol. 29, pp. 897-907, 1995.*
Holmstrom et al. The Plant Journal, vol. 6, pp. 749-758, 1994.*
Rozwadowski et al. Journal of Bacteriology, vol. 173, pp. 472-478, 1991.*
Rozwadowski et al., Journal of Bacteriology, vol. 173, pp. 472-478, 1991.
Kishitani et al. Plant Cell and Environment, vol. 17, pp. 89-95, 1994.*
Ko et al. Journal of Bacteriology, vol. 2, pp. 426-431, 1994.*
Zhao. et al. Journal of Plant Physiology, vol. 140, pp. 541-543, 1992.
Mehta Ashwin
Suntory Limited
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