Method for producing target substance

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...

Reexamination Certificate

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C424S184100, C424S185100, C424S190100, C424S192100, C424S234100, C424S278100, C435S004000, C435S007200, C435S007320, C435S029000, C435S106000, C435S115000, C435S243000, C435S252100, C435S252300, C435S440000, C436S086000, C436S089000, C562S561000, C562S562000

Reexamination Certificate

active

10234329

ABSTRACT:
In a method for producing a target substance by using a microorganism comprising culturing a microorganism having an ability to produce the target substance in a medium to produce and accumulate the target substance in the medium or cells of the microorganism and collecting the target substance from the medium or the cells of the microorganism, there are used, as the microorganism, a microorganism to which a methanol dehydrogenase gene is introduced, of which activities of hexulose phosphate synthase and phosphohexuloisomerase are enhanced and which is modified so that an ability to utilize methanol should be imparted or enhanced, and there is used a medium containing methanol as a carbon source.

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G.E. de Vries, et al., Journal of Bacteriology, vol. 174, No. 16, pp. 5346-5353, “Cloning, Expression, and Sequence Analysis of theBacillus MethanolicusC1 Methanol Dehydrogenase Gene”, Aug. 1992 (with abstract Accession No. M65004, 2 pages).
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