Method for producing stable glycosylated hemoglobin

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

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514 6, 436 67, C07K 1300, C07K 900

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active

051699370

ABSTRACT:
Methods of preparing glucitollysine-hemoglobin from a sample of glucohemoglobin containing stable and labile glucohemoglobins and for assaying for the presence of stable glucohemoglobin are disclosed, as is a diagnostic assay system useful for carrying out the methods.

REFERENCES:
Curtiss et al. (Oct. 1983). A novel method for generating region-specific monoclonal antibodies to modified proteins. J. Clin. Inv. 72:1427-1438.
Bisse et al. (Jul. 1982). Quantitation of glycosylated hemoglobin. Elimination of labile glycohemoglobin during sample hemolysis at pH 5. Diabetes 31:630-633.

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