Method for producing seeds and plants thereof involving an in vi

Details Method for producing seeds and plants thereof involving an in vi Method for producing seeds and plants thereof involving an in vi

1997-03-27

1998-11-24

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4353201, 435419, 800205, 800200, 800250, C12N 1500, C12N 1529, C12N 1582, A01H 1400

Patent

active

058405577

ABSTRACT:
A method for producing transgenic seeds and plants, which comprises isolating, from anthers, microspores in their uninucleate stage and removing tissue in which they are embedded, culturing the isolated microspores in a nutrient solution, transferring foreign genetic material into the isolated microspores to obtain transformed microspores, bringing about complete maturation of the transformed microspores to obtain transformed pollen grains in vitro, pollinating receiver plants with the transformed pollen grains and obtaining seeds from the pollinated receiver plants.

REFERENCES:
Negrutiu et al. Attempts to Transform for Kanamycin-Resistance in Mature Pollen of Tobacco. Biotechno. Ecol. Pollen, Proc. Int. Conf., Meeting Date 1985, Mulcahy et al., eds., 1986, Springer: New York.
Tanaka et al. Studies on Microspore Development in Liliaceous Plants III. Pollen Tube Development in Lily Pollins Cultured from the Uninucleate Microspore Stage. Plant and Cell Physiol. 22(1), 149-153, 1981.
Yasuo Ohta. High-efficiency genetic transformation of maize by a mixture of pollen and exogenous DNA. Proc. Natl. Acad. Sci. USA, vol. 83, pp. 715-719, Feb. 1986.
I. Potrykus, "Gene Transfer to Plants: Assessment of Published Approaches and Results", Annu. Rev. Plant Physiol. Plant Mol. Biol. 1991, 42: pp. 205-225.
Foster et al. "Comparative Morphology of Vascular Plants", 2nd Edition, San Francisco, W.H. Freeman et al., 1974, pp. 664-651.
Zambryski et al., "Ti Plasmid Vector for the Introduction of DNA Into Plant Cells Without Alteration of Their Normal Regeneration Capacity", The EMBO Journal vol. 2 No. 12, pp. 2143-2150 (1983).
E. Stoger et al., "Comparison of Different Techniques for Gene Transfer Into Mature and Immature Tobacco Pollen", Transgenic Research, vol. 1 (92) pp. 71-78.

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