Method for producing secretable glycosyltransferases and other g

Details Method for producing secretable glycosyltransferases and other g Method for producing secretable glycosyltransferases and other g

1994-03-10

1996-07-30

Ziska, Suzanne E.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

435 691, 435 701, 435 72, 435 97, 435100, 435101, 435193, 935 47, 935 48, 935 60, 935 70, 935 72, C12P 1918, C12P 2106, C12P 100, C12N 1552

Patent

active

055410839

ABSTRACT:
A method for genetically engineering cells to produce soluble and secretable Golgi processing enzymes instead of naturally occurring membrane-bound enzymes. Cells are genetically engineered to express glycosyltransferases which lack both a membrane anchor and a retention signal. The resulting altered enzyme becomes soluble and secretable by the cell without losing its catalytic activity. Secretion of the soluble glycosyltransferase by the cell provides for increased production and simplified recovery of glycosyltransferase.

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