Chemistry: molecular biology and microbiology – Vector – per se
Patent
1996-01-30
1998-07-07
Stanton, Brian R.
Chemistry: molecular biology and microbiology
Vector, per se
435 41, 435 691, 435 701, 4351723, 435 97, 435197, 435100, 435325, 935 48, 935 60, 935 70, 935 72, 935 47, 536 235, C12N 1563, C12N 1500, C12N 1554, C12N 1579
Patent
active
057767726
ABSTRACT:
A method for genetically engineering cells to produce soluble and secretable Golgi processing enzymes instead of naturally occurring membrane-bound enzymes. Cells are genetically engineered to express glycosyltransferases which lack both a membrane anchor and a retention signal. The resulting altered enzyme becomes soluble and secretable by the cell without losing its catalytic activity. Secretion of the soluble glycosyltransferase by the cell provides for increased production and simplified recovery of glycosyltransferase.
REFERENCES:
patent: 5032519 (1991-07-01), Paulson et al.
patent: 5541083 (1996-07-01), Paulson et al.
Gil et al Cell 41:249-258, 1985.
Smith et al J. Biol. Chem 252: 7294-7299, 1977.
Weinstein et al. J. Biol. Chem. 262: 17735-17743, 1987.
Adler Beverly
Browne Jeffrey K.
Colley Karen J.
Paulson James G.
Ujita-Lee Eryn
Schmuck Jill D.
Stanton Brian R.
The Regents of the University of California
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