Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Patent
1989-10-24
1991-07-16
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
4353201, 4351123, 4352402, 935 48, 935 60, 935 70, C12N 1500, C12N 1554, C12N 1563, C12N 1579
Patent
active
050325191
ABSTRACT:
A method for genetically engineering cells to produce soluble and secretable Golgi processing enzymes instead of naturally occurring membrane-bound enzymes. Cells are genetically engineered to express glycosyltransferases which lack both a membrane anchor and a r
REFERENCES:
Gil et al, Cell, vol. 41, 249-258 (1985).
Colley, K. J., Lee, E. U., Adler, B., Browne, J. K., and Paulson, J. C. (1989), Conversion of a Golgi Apparatus Sialyltransferase to a Secretory Protein by Replacement of the NH.sub.2 -Terminal Signal Anchor with a Signal Peptide, J. Biol. Chem. 264, 17619-17622.
Joziasse, D. H., Shaper, J. H., Van den Eijnden, D. H., Van Tunen, A. J., and Shaper, N. L. (1989), Bovine .alpha.1.fwdarw.3-Galactosyltransferase: Isolation & Char. of a cDNA Clone, J. Biol. Chem. 264, 14290-14297.
Kukowska-Latallo, J., Larsen, R. D., Nair, R. P., and Lowe, J. B. (1990), A Cloned Human cDNA Det. Exp. of a Mouse Stage-Specific . . . Genes Dev. 4, 1288-1303.
Larsen, R. D., Rajan, V. P., Ruff, M. M., Kukowska-Latallo, J., Cummings, R. D., and Lowe, J. B. (1989), Isolation of a cDNA . . . Proc. Natl. Acad. Sci., U.S.A. 86, 8227-8231.
Larsen, R. D., Ernst, L. K., Nair, R. P. and Lowe, J. B. (1990), Molecular Cloning, Sequence, and Expression . . . Proc. Natl. Acad. Sci., U.S.A. 87, 6674-6678.
Nakazawa, K., Ando, T., Kimura, T., and Narimatsu, H. (1988), Cloning and Sequencing of a Full-Length cDNA . . . J. Biochem. (Tokyo), 104, 165-168.
Narimatsu, H., Sinha, S., Brew, K., Okayama, H. and Quasba, P. K. (1986), Cloning and Sequencing of cDNA of Bovine . . . Proc. Natl. Acad. Sci., U.S.A. 83, 4720-4724.
Paulson, J. C., Weinstein, J., Ujita, E. U., Riggs, K. J., and Lai, P.-H. (1987), The Membrane Binding Domain of a Rat Liver . . . Biochem. Soc. Trans. 15, 618-620.
Shaper, N. L., Shaper, J. H., Meuth, J. L., Fox, J. L., Chang, H., Kirsch, I. R., and Hollis, G. F. (1986), Bovine Galactosyltransferase . . . Proc. Natl. Acad. Sci., U.S.A. 83, 1573-1577.
Shaper, N. L., Hollis, G. F., Douglas, J. G., Kirsch, I. R. and Shaper, J. J. H. (1988), Characterization of the Full Length cDNA for Murine .beta.1,4-Galactosyltransferase . . . J. Biol. Chem. 263, 10420-10428.
Weinstein, J., Lee, E. U., McEntee, K., Lai, P.-H. and Paulson, J. C. (1987), Primary Structure of .beta.-Galactoside . . . J. Biol. Chem. 262, 17735-17743.
Yamamoto, F., Marken, J., Tsuji, T., White, T., Clausen, H. and Hakomori, S. (1990), Cloning and Characterization of DNA Complementary . . . J. Biol. Chem. 265, 1146-1151.
Yamamoto, F., Clausen, H., White, T., Marken, J., and Hakomori, S. (1990), Molecular Genetic Basis of the . . . Nature 345, 229-233.
Paulson, J. C. and Colley, K. J. (1989), Glycosyltransferases-Structure, Localization and Control of Cell . . . Journal of Biol. Chem., vol. 264, No. 30, Oct. 25, pp. 17615-17618.
Tetrahdedron Reports, 1989, "Enzyme-Catalyzed Synthesis of Carbohydrates", Eric J. Toone et al.
Ann. Rev. Cell Biol., 1988, 4:257-88, "Regulation of Protein Export from the Endoplasmic Reticulum", John K. Rose and Robert W. Doms, pp. 257-288.
The Journal of Biological Chemistry, vol. 257, No. 22, Nov. 25 issue, pp. 13835-13844, 1982, "Purification of a Gal.beta.1 4GlcNAc . . . to Homogeneity from Rat Liver", J. Weinstein, U. deSouza-e Silva, and J. C. Paulson.
Adler Beverly
Browne Jeffrey K.
Colley Karen J.
Paulson James C.
Ujita-Lee Eryn
Nolan S. L.
Schwartz Richard A.
The Regents of the Univ. of California
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