Method for producing secretable glycosyltransferases and other G

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

4353201, 4351123, 4352402, 935 48, 935 60, 935 70, C12N 1500, C12N 1554, C12N 1563, C12N 1579

Patent

active

050325191

ABSTRACT:
A method for genetically engineering cells to produce soluble and secretable Golgi processing enzymes instead of naturally occurring membrane-bound enzymes. Cells are genetically engineered to express glycosyltransferases which lack both a membrane anchor and a r

REFERENCES:
Gil et al, Cell, vol. 41, 249-258 (1985).
Colley, K. J., Lee, E. U., Adler, B., Browne, J. K., and Paulson, J. C. (1989), Conversion of a Golgi Apparatus Sialyltransferase to a Secretory Protein by Replacement of the NH.sub.2 -Terminal Signal Anchor with a Signal Peptide, J. Biol. Chem. 264, 17619-17622.
Joziasse, D. H., Shaper, J. H., Van den Eijnden, D. H., Van Tunen, A. J., and Shaper, N. L. (1989), Bovine .alpha.1.fwdarw.3-Galactosyltransferase: Isolation & Char. of a cDNA Clone, J. Biol. Chem. 264, 14290-14297.
Kukowska-Latallo, J., Larsen, R. D., Nair, R. P., and Lowe, J. B. (1990), A Cloned Human cDNA Det. Exp. of a Mouse Stage-Specific . . . Genes Dev. 4, 1288-1303.
Larsen, R. D., Rajan, V. P., Ruff, M. M., Kukowska-Latallo, J., Cummings, R. D., and Lowe, J. B. (1989), Isolation of a cDNA . . . Proc. Natl. Acad. Sci., U.S.A. 86, 8227-8231.
Larsen, R. D., Ernst, L. K., Nair, R. P. and Lowe, J. B. (1990), Molecular Cloning, Sequence, and Expression . . . Proc. Natl. Acad. Sci., U.S.A. 87, 6674-6678.
Nakazawa, K., Ando, T., Kimura, T., and Narimatsu, H. (1988), Cloning and Sequencing of a Full-Length cDNA . . . J. Biochem. (Tokyo), 104, 165-168.
Narimatsu, H., Sinha, S., Brew, K., Okayama, H. and Quasba, P. K. (1986), Cloning and Sequencing of cDNA of Bovine . . . Proc. Natl. Acad. Sci., U.S.A. 83, 4720-4724.
Paulson, J. C., Weinstein, J., Ujita, E. U., Riggs, K. J., and Lai, P.-H. (1987), The Membrane Binding Domain of a Rat Liver . . . Biochem. Soc. Trans. 15, 618-620.
Shaper, N. L., Shaper, J. H., Meuth, J. L., Fox, J. L., Chang, H., Kirsch, I. R., and Hollis, G. F. (1986), Bovine Galactosyltransferase . . . Proc. Natl. Acad. Sci., U.S.A. 83, 1573-1577.
Shaper, N. L., Hollis, G. F., Douglas, J. G., Kirsch, I. R. and Shaper, J. J. H. (1988), Characterization of the Full Length cDNA for Murine .beta.1,4-Galactosyltransferase . . . J. Biol. Chem. 263, 10420-10428.
Weinstein, J., Lee, E. U., McEntee, K., Lai, P.-H. and Paulson, J. C. (1987), Primary Structure of .beta.-Galactoside . . . J. Biol. Chem. 262, 17735-17743.
Yamamoto, F., Marken, J., Tsuji, T., White, T., Clausen, H. and Hakomori, S. (1990), Cloning and Characterization of DNA Complementary . . . J. Biol. Chem. 265, 1146-1151.
Yamamoto, F., Clausen, H., White, T., Marken, J., and Hakomori, S. (1990), Molecular Genetic Basis of the . . . Nature 345, 229-233.
Paulson, J. C. and Colley, K. J. (1989), Glycosyltransferases-Structure, Localization and Control of Cell . . . Journal of Biol. Chem., vol. 264, No. 30, Oct. 25, pp. 17615-17618.
Tetrahdedron Reports, 1989, "Enzyme-Catalyzed Synthesis of Carbohydrates", Eric J. Toone et al.
Ann. Rev. Cell Biol., 1988, 4:257-88, "Regulation of Protein Export from the Endoplasmic Reticulum", John K. Rose and Robert W. Doms, pp. 257-288.
The Journal of Biological Chemistry, vol. 257, No. 22, Nov. 25 issue, pp. 13835-13844, 1982, "Purification of a Gal.beta.1 4GlcNAc . . . to Homogeneity from Rat Liver", J. Weinstein, U. deSouza-e Silva, and J. C. Paulson.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for producing secretable glycosyltransferases and other G does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for producing secretable glycosyltransferases and other G, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for producing secretable glycosyltransferases and other G will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-131279

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.