Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Patent
1985-08-16
1988-01-05
Martinell, James
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
435 41, 435118, 435119, 435121, 435122, 435132, 43524054, 43524048, C12P 766, C12P 100, C12P 1716, C12P 1718, C12P 1710, C12P 1712, C12P 700, C12N 500, C12N 502
Patent
active
047176644
ABSTRACT:
This invention provides for a method for producing secondary metabolites of higher plants, such as Lithospermum erythrorhizon, Coptis japonica, and Nicotinia tabacum, by suspension cultures of a mass of undifferentiated cells (callus). The cultures are carried out in at least two stages in liquid media. The first stage culturing is carried out in a liquid medium conventionally used for the tissue culture of plants, which contains indispensably inorganic substances and carbon sources and, additionally, phytohormones, vitamins and/or amino acids. While, the second stage culturing is carried out in another liquid medium, of which the concentration of at least one of the constituents is substantially varied, namely decreased or increased, from the first stage liquid medium. According to such two stage liquid medium cultures, the productivity of the second metabolites, such as shikonin, berberine and nicotine, are significantly increased, even though the rate of cell growth in the second stage is relatively low.
REFERENCES:
Tabata: in Plant Tissue Culture and its Bio-Technological Application, Barz et al. (ed.), Springer-Verlag, 1977, pp. 3-16.
Zenk et al.: in Plant Tissue Culture and its Bio-Technological Application, Barz et al. (ed.), Springer-Verlag, 1977, pp. 27-43.
Yamada et al.: Chem. Abstr. 95:30273v (1981) of Phytochemistry 20: 545 (1981).
Yadrov et al.: Chem. Abstr. 89:145697e (1978) of Restit. Res. 14: 408 (1978).
Manual of the Vascular Flora of the Carolinas, Radford et al., 1968, The University of North Carolina Press, Chapel Hill, N.C., pp. 925 and 935.
Phytochemistry, vol. 13, p. 927 (1974) Abstract only, Tabata et al.
Phytochemistry, vol. 16, p. 1183 (1977) Abstract only, Mizukami et al.
Phytochemistry, vol. 17, p. 95--(1978) Abstract only, Mizukami et al.
Pharmacological Journal, vol. 95, p. 1376, Tabata et al. (1975) Abstract only.
Chem. Abst., vol. 93, Oct. 1980, p. 414, Item 146587p., Kato et al.
Kato, J. Ferment. Technol. (1980), vol. 58(4), pp. 373-382.
Hara Yasuhiro
Suga Chuzo
Martinell James
Mitsui Petrochemical Industries Ltd.
LandOfFree
Method for producing secondary metabolites of plants does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for producing secondary metabolites of plants, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for producing secondary metabolites of plants will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-8867