Method for producing S-adenosyl-L-homocysteine hydrolase

Details Method for producing S-adenosyl-L-homocysteine hydrolase Method for producing S-adenosyl-L-homocysteine hydrolase

1984-07-25

1986-09-02

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435822, 435829, 435830, 435843, 435850, 435867, 435872, 435874, 435831, 435863, 435886, C12N 914, C12R 101, C12R 105, C12R 106, C12R 1065, C12R 1465, C12R 115, C12R 120, C12R 132, C12R 129, C12R 1365, C12R 138

Patent

active

046096264

ABSTRACT:
A method for producing S-adenosyl-L-homocysteine hydrolase, which comprises cultivating a microorganism having the ability to produce S-adenosyl-L-homocysteine hydrolase within its cells in a nutrient medium to accumulate said hydrolase in the cells, said microorganism being a bacterium belonging to the genera Alcaligenes, Pseudomonas, Acinetobacter, Arthrobacter, Enterobacter, Rhodopseudomonas, Agrobacterium, Micrococcus, Corynebacterium, Brevibacterium, Chromobacterium, Xanthomonas, Flavobacterium, Cellulomonas, Azotobacter and Protaminobacter, or an actinomycete belonging to the genera Streptomyces, Mycobacterium, Nocardia, Streptoverticillium, Micromonospora, Micropolyspora, Streptosporangium and Microellobosporia; and then recovering S-adenosyl-L-homocysteine hydrolase from the cells.

REFERENCES:
Journal of Bacteriology, vol. 112, No. 1, pp. 569-575, Oct. 1982.
Canadian Journal of Biochemistry, vol. 53, pp. 312-319 (1975).
Eur. J. Biochemistry, vol. 141, pp. 385-392 (1984).
Guranowski, et al., "Adenosylhomocysteinase from Yellow Lupin Seeds," Eur. J. Biochem., 80, (1977), pp. 517-523.
Fujioka, et al., "S-Adenosylhomocysteine Hydrolase from Rat Liver," J. Biol. Chem., vol. 256, No. 4, (1981), pp. 1631-1635.

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