Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1995-06-05
2000-10-24
Scheiner, Laurie
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 6, 435325, 4352351, 43525233, 43525411, 435440, 4352523, 4242171, C12N 1543
Patent
active
06136570&
ABSTRACT:
The present invention relates to methods for producing RNA virus cDNA, methods for producing viable, RNA virus and viable, RNA virus produced by those methods. The invention also related to a novel RNA virus cDNA, recombinant DNA molecules containing that cDNA and hosts transformed with those recombinant cDNA molecules. This invention further related to novel methods for screening for variants of a strain 3 poliovirus. This invention also related to methods for increasing the attenuation of a strain poliovirus.
This invention provides a vaccine useful for immunizing a subject, for example a human, against infectious poliovirus, wherein the vaccine comprises an effective amount of an RNA virus, produced by transforming a suitable host cell with a recombinant nucleic acid sequence which encodes for the virus: culturing the host cell under conditions which permit the production of virus: and isolating the virus so produced, effective to immunize the subject, and a suitable carrier. Further provided to this invention is a method of immunizing a subject such as a human against infectious poliovirus, wherein the method comprises administering the subject a suitable dose of the vaccine described hereinabove.
REFERENCES:
patent: 4719177 (1988-01-01), Baltimore et al.
Auld V.J. et al., "A Neutral Amino Acid Change In Segment IIS4 Dramatically Alters The Gating Properties Of The Voltage-Dependent Sodium Channel", Proc. Natl. Acad. Sc. (USA) Jan. 1990, vol. 87, pp. 323-327 (Exhibit 3).
Boslego J.W., "Gonorrhea Vaccines", Vaccine and Immunotherapy S.J. Cryz Edt., 1990, Pergamon Press, pp. 211-223 (Exhibit 4).
Bowie J.U. et al., "Deciphering The Message In Protein Sequences: Tolerance to Amino Acid Substitutions", Science, 1990, vol. 247, pp. 1306-1310 (Exhibit 5).
Davis, et al., Microbiology, Third Edition, "Picornavirus" Chapter 57, 1980, pp. 1096-1108, Harper & Row, Publishers, PA (Exhibit 6).
DeBorde D.C., et al., "Resolution of a Common RNA Sequencing Ambiguity by Terminal Deoxynucleotidyl Transferase", Analytical Biochemistry, 1986, vol. 157, pp. 275-282.
Ellis R.W., "New Technology for Making Vaccines", Vaccines, Plotkin & Mortimer Edt., W.B. Saunders Co., 1988. (Exhibit 8).
Kohara M., et al., "An Infectious cDNA Clone of the Poliovirus Sabin Strain Could be used as a Stable Repository and Inoculum for the oral Polio Live Vaccine", Virology, 1986, vol. 151.
Kuhn R.J., et al., Expression of the Poliovirus Genome from Infectious cDNA is Dependent upon Arrangements of Eukaryotic and Prokaryotic Sequences in Recombinant Plasmids, Virology, 1987, vol. 157, pp. 560-564 (Exhibit 10).
Kumar V. et al., "Amino Acid Variations at a Single Residue in an Autoimmune Peptide Profoundly Affect its Properties: T-cell Activation, Major Histocompatibility Complex Binding, and Ability to Block Experimental Allergic Encephalomyelitis", Proc. Natl. Acad. Sci. USA (Feb., 1990), vol. 87, pp. 1337-1341 (Exhibit 11).
Roos R.P., et al., "Infectious cDNA Clones of the DA Strain of Theiler's Murine Encephalomyelitis Virus", Journal of Virology (Dec. 1989), pp. 5492-5496 (Exhibit 12).
Saltarelli M. et al., "Nucleotide Sequence and Transcriptional Analysis of Molecular Clones of CAEV Which Generate Infectious VIrus", Virology, (1990), vol. 179, pp. 347-364 (Exhibit 13).
Stanway G. et al., "The Nucleotide Sequence of Poliovirus Type 3 Leon 12 a.sub.1 b: Comparison with Poliovirus Type 1", Nucleic Acids Research, (1983), vol. 11, No. 16, pp. 5629-5643.
Toyoda H. et al., "Complete Nucleotide Sequences of all three Poliovirus Serotype Genomes", J. Mol. Biol., (1984), vol. 174, pp. 561-584 (Exhibit 15).
Verma I.M. et al., "Reverse Transcriptase", The Enzymes, (1981), vol. 14, pp. 87-103 (Exhibit 16).
Racaniello Vincent
Tatem Joanne Marie
Weeks-Levy Carolyn L.
Scheiner Laurie
The Trustees of Columbia University in the City of New York
White John P.
LandOfFree
Method for producing RNA viruses from cDNA does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for producing RNA viruses from cDNA, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for producing RNA viruses from cDNA will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1962320