Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound other than saccharide containing...
Patent
1990-02-15
1992-11-17
Marx, Irene
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound other than saccharide containing...
435255, 4351721, 435244, C12P 0500, C12N 1165, C12N 1500, C12N 138
Patent
active
051643030
ABSTRACT:
Strains of yeast of the species Candida famata are disclosed which can produce 10 grams of riboflavin per liter in 6 days, and in particular, strains identified by ATCC Accession Nos. 20849 and 20850. Riboflavin yields of more than 20 grams per liter in 200 hours have been achieved. Strains of the present invention have increased sensitivity to iron inhibition of flavinogenesis and have enhanced riboflavin production per cell at increased iron concentrations in the fermentation medium. The invention also is directed toward a process for selecting improved microorganisms which are resistant to inhibition of growth by depleted medium. Such selected mircoorganisms are then tested for riboflavin overproduction. The present invention is also directed toward a selection process in which mutated microorganisms are cultured in the presence of tubercidin, a purine analog. Mutant strains resistant to tubercidin are then tested for riboflavin over-production. The present invention also includes a process for producing riboflavin by culturing strains of riboflavin overproducing microorganims in a fermentation medium. Increased riboflavin production can be obtained by elevated iron concentrations in the nutrient medium. The fermentation is conducted and after cell growth levels off, riboflavin is recovered from the fermentation medium.
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Burdzinski Linda A.
Heefner Donald L.
Weaver Craig A.
Yarus Michael J.
Marx Irene
ZeaGen, Inc.
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