Method for producing recombinant human myoglobin

Details Method for producing recombinant human myoglobin Method for producing recombinant human myoglobin

1995-09-29

1999-03-30

Carlson, Karen

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

43525233, 4353201, 536 235, 530385, C12P 2106, C12N 120, C07H 1700, C07K 14805

Patent

active

058887660

ABSTRACT:
The present invention relates to a method for producing human myoglobin by inserting a DNA sequence comprising a human myoglobin gene into a tryptophan promoter-containing vector, followed by making E. coli with the thus-obtained expression vector express a holo-type human myoglobin. Using this method, it is possible to directly and efficiently produce a heme-structured, holo-type human myoglobin by gene recombination technology.

REFERENCES:
Georgiou 1988 Alche J. 34(8): 1233-1248.
Spinger et al 1987 P.N.A.S. 84: 9861-8965.
Furman et al 1987 Bio/Technology 5:1047-1050.
Guillemette et al. 1991 Prot. Eng. 4(5):585-592.
Grojean 1982 Gene 18: 199-209.
Eiko Akaboshi, Cloning of the Human Mycoglobin Gene, Elsevier Publications, vol. 33, pp. 241-249, 1985.
Varadarajan et al, Cloning, Expression in Escherichia coli, and Reconstruction of Human Mycoglobin, Proc. Natl. Acad. Sci., vol. 82, pp. 5681-5684, Sep. 1985.

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