Method for producing purified recombinant Haemophilus influenzae

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification

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530412, 530413, C07K 14285

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active

057081492

ABSTRACT:
Purified and isolated nucleic acid is provided which encodes a transferrin receptor protein of a strain of Haemophilus or a fragment or an analog of the transferrin receptor protein. The nucleic acid sequence may be used to produce peptides free of contaminants derived from bacteria normally containing the Tbp1 or Tbp2 proteins for purposes of diagnostics and medical treatment. Furthermore, the nucleic acid molecule may be used in the diagnosis of infection. Also provided are recombinant Tbp1 or Tbp2 and methods for purification of the same. Live vectors expressing epitopes of transferrin receptor protein for vaccination are provided.

REFERENCES:
patent: 5141743 (1992-08-01), Schryvers
Ferron, et al 1993. FEMO Microbiol. Letters 109: 159-166.
Schryvers, 1989. J. Med. Microbiol. 29: 121-130.
Sofer, et al. 1983. BioTechniques, "Designing an Optimal Chromatographic Purification Scheme for Proteins".
Stevenson, et al. 1992. Infect. and Immun. 60(6):2391-96.
Cornelissen, et al. 1992. J. Bact. 174(18): 5788-97.

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