Method for producing proteins tagged at the N- or C-terminus

Details Method for producing proteins tagged at the N- or C-terminus Method for producing proteins tagged at the N- or C-terminus

2006-08-22

2006-08-22

Celsa, Bennett (Department: 1639)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S091100, C435S320100, C530S350000, C536S023100, C536S025300, C536S025320

Reexamination Certificate

active

07094568

ABSTRACT:
The present invention relates to novel methods of producing proteins in which one or more domains are full length and correctly folded and which are each tagged at either the N- or C-terminus with one or more marker moieties and arrays containing such proteins, as well as the use of such proteins in arrays for rapid screening.

REFERENCES:
patent: 6087103 (2000-07-01), Burmer
patent: 6239209 (2001-05-01), Yang et al.
patent: 2002/0162622 (2002-11-01), Gut
patent: WO 99/11777 (1999-03-01), None
patent: WO 99/39210 (1999-08-01), None
patent: WO 99/51773 (1999-10-01), None
patent: WO 00/04382 (2000-01-01), None
patent: WO 00/09654 (2000-02-01), None
patent: WO 00/09654 (2000-06-01), None
patent: WO 01/04265 (2001-01-01), None
patent: WO 01/04265 (2001-04-01), None
patent: WO 01/57198 (2001-08-01), None
patent: WO 01/57198 (2002-02-01), None
Bordini, E., and Hamdan, M., “Investigation of Some Covalent and Noncovalent Complexes by Matrix-assisted Laser Desorption/Ionization Time-of-flight and Electrospray Mass Spectrometry,”Rapid Commun. Mass Spectrom. 13:1143-1151, John Wiley & Sons, Ltd. (1999).
Cai, J., et al., “Functional Expression of Multidrug Resistance Protein 1 inPichia pastoris,” Biochemistry 40:8307-8316, American Chemical Society (Jun. 2001).
DeRisi, J., et al., “Use of a cDNA microarray to analyse gene expression patterns in human cancer,”Nat. Genet. 14:457-460, Nature Publishing Co. (1996).
Doellgast, G.J., et al., “Sensitive Enzyme-Linked Immunosorbent Assay for Detection of Clostridium botulinum Neurotoxins A, B, and E Using Signal Amplification via Enzyme-Linked Coagulation Assay,”J. Clin. Microbiol. 31:2402-2409, American Society for Microbiology (1993).
Giuliani, C.D., et al., “Expression of an active recombinant lysine 49 phospholipase A2myotoxin as a fusion protein in bacteria,”Toxicon 39:1595-1600, Elsevier Science Ltd. (2001).
Hara, H., et al., “Molecular cloning and functional expression analysis of a cDNA for human hepassocin, a liver-specific protein with hepatocyte mitogenic activity,”Biochim. Biophys. Acta 1520:45-53, Elsevier Science B.V. (2001).
Rhodes, N., et al., “Expression and Purification of Active Recombinant ATM Protein from Transiently Transfected Mammalian Cells,”Prot. Express. Purif. 22:462-466, Academic Press, Inc. (Aug. 2001).
Staudinger, J., et al., “Interactions among Vertebrate Helix-Loop-Helix Proteins in Yeast Using the Two-hybrid System,”J. Biol. Chem. 268:4608-4611, The American Society for Biochemistry and Molecular Biology, Inc. (1993).
Vojtek, A.B., et al., “Mammalian Ras Interacts Directly with the Serine/Threonine Kinase Raf,”Cell 74:205-214, Cell Press (1993).
Walker, E.A., et al., “Functional Expression, Characterization, and Purification of the Catalytic Domain of Human 11-β-Hydroxysteroid Dehydrogenase Type 1,”J. Biol. Chem . 276:21343-21350, The American Society for Biochemistry and Molecular Biology, Inc. (2001).
Zwicker, N., et al., “Strep-tag® II for One-Step Affinity Purification of Active bHLHzip Domain of Human c-Myc,”BioTechniques 27:368-370 and 372-375, Eaton Publishing Co. (1999).
International Search Report for International Patent Application No. PCT/GB01/03693, mailed Nov. 7, 2002.

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