Method for producing proteins and polypeptides using ubiquitin f

Details Method for producing proteins and polypeptides using ubiquitin f Method for producing proteins and polypeptides using ubiquitin f

1988-04-07

1992-07-21

Peet, Richard C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 691, 435 201, 435 711, 435 91, 435171, 4351721, 4351723, 4352402, 435254, 4353201, 435911, 435940, 536 27, 935 6, 935 9, 935 10, 935 22, 935 23, 935 24, 935 33, 935 34, 935 37, 935 42, 935 48, 935 49, 935 51, 935 59, 935 60, 935 61, 935 66, 935 68, 935 69, 935 70, 935 71, C12P 2100, C12N 115, C12N 119, C12N 510, C12N 1509, C12N 1511, C12N 1579

Patent

active

051322137

ABSTRACT:
A method of designing or modifying protein structure at the protein or genetic level to produce specified amino-termini in vivo is described. The method is used to alter the metabolic stability and other properties of the protein or, alternatively, to artificially generate authentic amino-termini in proteins produced through artificial means. The method is based upon the introduction of the use of artificial ubiquitin-protein fusions, and the discovery that the in vivo half-life of a protein is a function of the amino-terminal amino acid of the protein.

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