Method for producing protein in a cell-free system

Details Method for producing protein in a cell-free system Method for producing protein in a cell-free system

1994-08-25

1997-01-14

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435 691, C12P 2100

Patent

active

055938566

ABSTRACT:
A method for producing protein in a cell-free system uses cell-free extract required for protein production, extracted from a biological cell. According to the present invention, a wide range of proteins can be produced economically and efficiently even in a semi-continuous operation mode, by regenerating energy source, increasing operation time of the reactor with porous solid material and using selective protein isolating means having high specific affinity to a desired protein. Thus, problems appearing in the living cell (in vivo) system due to the absence of post-translational processing can be avoided and the total production amount can be greatly increased due to prolonged operation time of the reactor and increased protein productivity.

REFERENCES:
patent: 5324637 (1994-06-01), Thompson et al.
Eckert et al., Animal Physiology, W. H. Freeman & Co., NY, 1983, p. 274.
Endo et al., J. Biotechnol. 25:221-230 (1992).
Kigawa et al., J. Biochem. 110:166-168 (1991).
Nakano et al., Biosci. Biotech. Biochem. 58:631-634 (1994).
Yoshizawa et al., Nucleic Acids Res. 22:2217-2221 (Jun. 1994).
Millipore 1992/1993 Pharmaceutical Process Filtration Catalog, Millipore Corp., Marlborough, MA.

Affiliated with

Also associated with

Rating

Method for producing protein in a cell-free system does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for producing protein in a cell-free system, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for producing protein in a cell-free system will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1387415