Method for producing polyunsaturated long-chain fatty acids...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

Reexamination Certificate

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Details

C435S069100, C435S091100, C435S320100, C435S252300, C536S023200, C554S008000, C800S278000, C800S291000

Reexamination Certificate

active

07871804

ABSTRACT:
The invention relates to a method for producing polyunsaturated fatty acids in an organism, according to which nucleic acids coding for polypeptides with an acyl-CoA:lysophospholipid-acyltransferase activity are introduced into the organism. Advantageously, the nucleic acid sequences can be expressed in the transgenic organism optionally together with other nucleic acid sequences coding for polypeptides of the fatty acid or lipid metabolism. The invention also relates to the inventive nucleic acid sequences, nucleic acid constructs containing the inventive nucleic acid sequences, vectors containing the inventive nucleic acid sequences and/or the nucleic acid constructs, and transgenic organisms containing the nucleic acid sequences, nucleic acid constructs and/or vectors. The invention further relates to oils, lipids and/or fatty acids produced according to the inventive method, and to the use of the same.

REFERENCES:
patent: 2004/0034888 (2004-02-01), Liu et al.
patent: 2006/0168687 (2006-07-01), Renz et al.
patent: 2006/0174376 (2006-08-01), Renz et al.
patent: 2785911 (2000-05-01), None
patent: WO-00/18889 (2000-04-01), None
patent: WO-2004/076617 (2004-09-01), None
patent: WO-2004/087902 (2004-10-01), None
patent: WO 2004/087902 (2004-10-01), None
Broun et al., Catalytic plasticity of fatty acid modification enzymes underlying chemical diversity of plant lipids. Science, 1998, vol. 282: 1315-1317.
Devos et al., Practical limits of function prediction. Proteins: Structure, Function, and Genetics. 2000, vol. 41: 98-107.
Guo et al., Protein tolerance to random amino acid change. PNAS., 2004, vol. 101 (25): 9205-9210.
Kisselev L., Polypeptide release factors in prokaryotes and eukaryotes: same function, different structure. Structure, 2002, vol. 10: 8-9.
Whisstock et al., Prediction of protein function from protein sequence. Q. Rev. Biophysics., 2003, vol. 36 (3): 307-340.
Wishart et al., A single mutation converts a novel phosphotyrosine binding domain into a dual-specificity phosphatase. J. Biol. Chem., 1995, vol. 270(45): 26782-26785.
Seffernick et al., Melamine deaminase and Atrazine chlorohydrolase: 98 percent identical but functionally different. J. Bacteriol.,2001, vol. 183 (8): 2405-2410.
Witkowski et al., Conversion of b-ketoacyl synthase to a Malonyl Decarboxylase by replacement of the active cysteine with glutamine. Biochemistry, 1999, vol. 38: 11643-11650.
Chica et al., Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design. Curr. Opi. Biotechnol., 2005, vol. 16: 378-384.
Current Protocols in Molecular Biology, 1993, 2.10.1-2.10.16, Copyright 2000 by John Wiley & Sons, Inc.
Kimchi-Sarfaty et al., A “Silent” polymorphism in the MDR1 gene changes substrate specificty. Science, 2007, vol. 315: 525-528.
Nackley et al., Human Caechol-O-Methytransferase haplotypes modulate protein expression by altering mRNA secondary structure. Science, 2006, vol. 314: 1930-1933.
Sauna et al., Silent polymorhisms speak: How they affect pharmacogenomics and the treatment of cancer. Cancer Res., 2007, vol. 67(20): 9609-9612.
Sen et al., Developments in directed evolution for improving enzyme functions. Appl. Biochem. Biotechnol., 2007, vol. 143: 212-223.
Domergue, F. et al., “Relief for fish stocks: oceanic fatty acids in transgenic oilseeds”, Trends in Plant Science, 2005, vol. 10, No. 3, pp. 112-116.
“Lysophosphatidic acid acyltransferase, putative”, UniProtKB Database Accession No. Q337H3, Jan. 24, 2006.
Yamashita, A. et al., “ATP-independent fatty acyl-coenzyme A synthesis from phospholipid”, The Journal of Biological Chemistry, 2001, vol. 276, No. 29, pp. 26745-26752.
Dahlqvist, A. et al., “Phospholipid: diacylglycerol acyltransferase: an enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants”, PNAS, 2000, vol. 97, No. 12, pp. 6487-6492.
Yamashita, A. et al., “Acyltransferases and transacylases involved in fatty acid remodeling of phospholipids and metabolism of bioactive lipids in mammalian cells”, J. Biochem., 1997, vol. 122, pp. 1-16.

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