Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1998-01-28
2000-10-31
Achutamurthy, Ponnathapu
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4353201, 43525422, 435196, C12P 2106, C12N 1500, C12N 916, C12N 114
Patent
active
061400777
ABSTRACT:
A method for producing phytase using a Candida boidinii transformant, by which phytase is secreted to liquid medium without substantially increasing the number of the transformant cells is disclosed. In this method, cells of a Candida boidinii transformant which is transformed with a recombinant vector comprising a promoter inducible with methanol and a gene located downstream of said promoter and operably linked to said promoter, which gene encodes a polypeptide having phytase activity, are cultured in a liquid culture medium containing phosphoric acid or a phosphate compound in an amount effective of restricting proliferation of said cells of Candida boidinii transformant such that the amount of said cells of Candida boidinii transformant is not substantially increased, while adding methanol to the culture medium, so as to make said cells secrete said phytase.
REFERENCES:
patent: 5750372 (1998-05-01), Sakai et al.
Sakai, Y. et al., Appl. Microbiol. Biotechnol., vol. 42, pp. 860-864, 1995.
Sakai, Y. et al., Appl. Environ. Microbiol., vol. 53, No. 8, pp. 1812-1818, Aug. 1987.
Egli, T. et al., J. Gen. Microbiol., vol. 123, p. 365-369, 1981.
XP-002067635, Chemical Abstracts 164352d, vol. 128, No. 14.
XP-002067634, Abstract of the Paper of the 196.sup.th Meeting of the American Chemical Society, p. mbtd105.
Kato Nobuo
Mochizuki Daisuke
Nakamura Takeshi
Sakai Yasuyoshi
Suzuki Tadashi
Achutamurthy Ponnathapu
Mitsui Chemicals Inc.
Tung Peter P.
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