Method for producing phalaenosis clone plants through root tip c

Details Method for producing phalaenosis clone plants through root tip c Method for producing phalaenosis clone plants through root tip c

1997-04-02

1999-02-02

Wax, Hobert A.

Plant husbandry

Process

800200, 800205, 800DIG62, 800220, 435420, 435430, A01B 7900, A01G 100, A01H 104, A01H 102

Patent

active

058649859

ABSTRACT:
Disclosed is a method for producing Phalaenopsis orchid clone plants, which comprises cutting the tip of a growing root of a Phalaenopsis orchid that has been prepared under sterile conditions at a length of from 1 to 5 mm to prepare a root tip for culture having said predetermined length, cultivating said root tip in a PLB-inducing medium under sterile conditions to thereby induce PLB therein, further cultivating said PLB in a propagating medium, and then re-differentiating said PLB in a re-differentiation medium. The method is practicable and stably given PLB of Phalaenopsis orchids.

REFERENCES:
Yoneda et al. (1988). Bull. Coll. Agr. & Vet. Med., Nihon Univ., No. 45, pp.: 191-196, 1988.
Michio Tanaka, et al., American Orchid Society, vol. 45, pp. 1022-1024, Nov. 1976, "Plantlet Formation by Root-Tip Culture in Phalaenopsis".
Derwent Abstracts, AN 95-008999, JP6292477, Oct. 21, 1994.
WPI Abstracts, AN 95-009000, JP6292478, Oct. 21, 1994.
G. R. Price, et al., American Orchid Society Bulletin, vol. 53, No. 10, 1984, "Sources of Orchid Protoplasts for Fusion Experiments", (Abstract Only).
Tian-Su Zhou, Plant Cell Reports, vol. 15, 1995, pp. 181-185, "In Vitro Culture of Doritaenopsis: Comparison Between Formation of the Hyperhydric Protocorm-Like Body (PLB) and the Normal PLB".
T. W. Yam, et al., Lindleyana, vol. 6, No. 3, pp. 151-153, 1991, "Root-Tip Culture of Several Native Orchids of Hong Kong".
Derwent Abstracts, AN 97-252898, JP9084477, Mar. 31, 1997.
M. Kobayashi, et al., Tochigi Pref. Agr. Exp. Stn., pp. 664-665, Feb. 1990, "Studies on the Vegetative Propagation of Phalaenopsis Through Root-Tip Culture" (With English Translation).

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