Method for producing penicillinase and xylanase

Details Method for producing penicillinase and xylanase Method for producing penicillinase and xylanase

1989-08-30

1993-03-02

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

43525233, 4353201, C12N 924, C12N 121, C12N 1570

Patent

active

051908743

ABSTRACT:
A novel plasmid pXP102-3 which is constructed from Aeromonas sp. No. 212 chromosomal DNA fragment coding for production of xylanase and a vector plasmid pEAP2 carrying a DNA fragment coding for production and secretion of penicillinase. A novel microorganism, Escherichia coli HB101 (pXP102-3), containing the plasmid pXP102-3 and being capable of producing and secreting penicillinase and xylanase. A method for cultivation of the microorganism characterized in that it is cultured in a broth containing NaCl for 12 to 48 hours.

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