Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals
Patent
1998-05-29
1999-10-05
Achutamurthy, Ponnathapura
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
436523, 436524, 436525, 436526, 436527, 436528, 436529, 436530, 436531, 530333, 530334, 4352831, 4352861, G01N 33543, A61K 3800
Patent
active
059623381
ABSTRACT:
A method of using a reaction plenum in the presence or absence of paramagnetic beads to perform chemical syntheses resulting in separation and recovery of a final desired reaction product is provided. The reaction plenum comprises a reaction plate, a reaction vessel for mounting in the reaction plate, a reaction plate holder, a screw-like rod, a mounting block attached to the reaction plate holder for operably receiving the screw-like rod, a motor, a sonication region in a temperature controllable water bath and a magnetic separation region in the water bath. Paramagnetic beads having reaction sites are introduced into the reaction vessels along with the appropriate solvent and one or more reactants. The contents of the reaction vessel are sonicated, then moved to the magnetic separation region where the paramagnetic beads are tightly held against the reaction vessel while the solvent is aspirated off. Once the desired reaction product is attained, the reaction product is cleaved off the paramagnetic bead. In an alternative embodiment, chemical synthesis is performed in a similar manner in the absence of the paramagnetic beads.
REFERENCES:
patent: 3552536 (1971-01-01), Emary
patent: 3811623 (1974-05-01), Speer
patent: 3837805 (1974-09-01), Boucher
patent: 3915605 (1975-10-01), Natelson
patent: 4022579 (1977-05-01), Revillet et al.
patent: 4259290 (1981-03-01), Souvaniemi et al.
patent: 4582990 (1986-04-01), Stevens
patent: 4638032 (1987-01-01), Benner
patent: 4919807 (1990-04-01), Morton
patent: 5320944 (1994-06-01), Okada et al.
patent: 5380487 (1995-01-01), Choperena et al.
patent: 5446263 (1995-08-01), Eigen et al.
patent: 5496517 (1996-03-01), Pfost et al.
Fjeld, J.G. et al., J. Immunol. Methods, vol. 109, p. 1 (1988).
Miltenyi, S. et al. Cytometry, vol. 11, p. 231, (1990).
Padmanabhan, R., et al. Analytical Biochem., vol. 170, p. 341, (1988).
Treleaven, J.G. et al. Lancet, vol. 14, p. 70, (1984).
Achutamurthy Ponnathapura
Cohen Jerry
Erlich Jacob N.
Ponnaluri P.
Solid Phase Sciences Corp.
LandOfFree
Method for producing organic molecules on a paramagnetic bead does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for producing organic molecules on a paramagnetic bead, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for producing organic molecules on a paramagnetic bead will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1170910