Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1989-05-05
1992-09-29
Naff, David M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435217, 435212, 435215, C12N 964, C12N 948, C12N 972, C12N 968
Patent
active
051513595
ABSTRACT:
A method for the production of human tissue type plasminogen activator (tPa) using cells is disclosed. The method includes a supplementation of a p-aminomethyl benzoic acid derivative to a cell culture medium or a tPA producing medium and further an increase of osmotic pressure in the medium to 350 milliosmoles or more/liter. The invention provides a method for producing single-chain tPA in a high concentration and with a relatively small amount of double-chain tPA in the medium.
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Kusuhara Nobumi
Makiguchi Nobuyoshi
Miyahara Shyoichiro
Shindo Atsunori
Suzuki Maki
Meller Mike
Mitsui Toatsu Chemicals Incorporated
Naff David M.
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