Method for producing nucleoside-5'-phosphate ester

Details Method for producing nucleoside-5'-phosphate ester Method for producing nucleoside-5'-phosphate ester

1997-11-21

2000-01-18

Nashed, Nashaat

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435194, 435195, 4352523, 43525233, 536 232, C12P 1938

Patent

active

06015697&

ABSTRACT:
A method for producing nucleoside-5'-phosphate esters inexpensively and in high yields by phosphorylating a nucleoside with a phospahte group donor using an acid phosphatase having an increased affinity for the nucleoside and/or an increased temperature stability at a pH of pH 3.0 to 5.5, to produce a nucleoside-5'-phosphate ester. Mutant acid phosphatases having increased affinity for nucleosides and/or an enhanced temperature stability are also provided.

REFERENCES:
Abstract for Congress of the Society for Fermentation and Bioengineering, p. 356, Oct. 10, 1994, Y. Asano, et al., "Phosphorylation of Nucleosides with Pyrophosphate as a Phosphate Donor" (With English Translation).
Nippon Nogeikagaku Kaishi, vol. 69, p. 270 (20a10), Jul. 5, 1995, Y. Mihara, et al., "Enzymatic Phosphorylation Reaction of Nucleoside using Pyrophosphate as a Phosphate Donor" (With English Translation).

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