Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound having a 1-thia-4-aza-bicyclo
Reexamination Certificate
2007-07-10
2007-07-10
Lankford, Leon B. (Department: 1651)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound having a 1-thia-4-aza-bicyclo
C435S047000
Reexamination Certificate
active
10657199
ABSTRACT:
The present invention provides a method for producing a novel β-lactam antibiotic from a protoplast fusion strain. The method is to fermentatively culture the protoplast fusion strain ofPenicillium chrysogenumandCephalosporium acremonium. The ferment filtrate is isolated, lyophilized, and extracted by acetone or acetone/methanol. The extract is concentrated by decompression, and then analyzed by preparation type HPLC to isolate the active antibiotic compound.
REFERENCES:
Chang et al. “Protoplast fusion in industrial fungi” Developments Industrial Microbiol. (1982) 23: 21-29.
Chen Chin-Chu
Chen Ching-Nung
Chen Yen-Lien
Chen Yi-Hsuan
Chung Wei-Hui
Grape King Inc.
Hanley Susan
King Anthony
Lankford Leon B.
WPAT, P.C.
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